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Western blot analysis Cells were harvested in lysis buffer [50 mM Tris (pH 7.5), 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 1 mM EDTA, and 0.1% SDS] containing protease inhibitor cocktail (Sigma-Aldrich), and the protein concentrations were determined using the BCA Protein Assay (Thermo Fisher Scientific)

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