Multi-Species VMA (Vanillylmandelic Acid) ELISA Kit SF then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Mouse FE

Then a biotinylated detection antibody specific for Human CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Only those wells that contain Mouse FETUA

The concentration of Mouse ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Multi-Species VMA (Vanillylmandelic Acid) ELISA Kit SF then added a HRP-conjugated antibodyThe VMA (Vanillylmandelic Acid) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal VMA. During the reaction, Universal VMA in the sample or standard competes with a fixed amount of Universal VMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VMA. Excess conjugate and unbound sample or standard are washed away, and Avidin